Fluorescein diacetate vital staining for detecting viability of acid-fast bacilli in patients on antituberculosis treatment
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چکیده
منابع مشابه
Staining techniques for detection of acid fast bacilli: what hope does fluorescein-diacetate (FDA) vitality staining technique represent for the monitoring of tuberculosis treatment in resource limited settings
Despite the recent development of more sensitive technologies, diagnosis of tuberculosis in resource limited settings continues to rely on sputum smear microscopy. This is explained by the fact that smear microscopy is simple, inexpensive, and the most accessible tool in resource limited settings for tuberculosis diagnosis. Hot Ziehl-Neelsen (Z-N) is still used in laboratories of resource limit...
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To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...
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Tuberculosis is a disease of great importance in developing countries, such as India, where it has caused considerable morbidity and mortality. Sputum smear microscopy is the cornerstone in diagnosis of pulmonary tuberculosis, which is usually done using Ziehl–Neelsen (ZN) method (Vasanthakumari et al., 1986; Gupta et al., 2009; Chandrasekaran et al., 1991). The use of ZN Method in remote areas...
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We compared the sensitivity and specificity of a modified Ziehl-Neelsen (modified-ZN) staining method for acid-fast bacilli (AFB) with that of the standard Ziehl-Neelsen (standard-ZN) staining method, using culture results with Mycobacterium tuberculosis as the "gold standard." The sensitivity (72%; 101 of 140) of the modified-ZN staining method, which uses 0.3% carbol fuchsin, was significantl...
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A dual-staining procedure for acid-fast bacilli was found to have poor correlation with the Ziehl-Neelsen and auramine-rhodamine staining techniques.
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ژورنال
عنوان ژورنال: International Journal of Mycobacteriology
سال: 2016
ISSN: 2212-5531
DOI: 10.1016/j.ijmyco.2016.06.003